Rat SCF Antibody ELISA Kit

Product Detail

Format	96-well strip plate
Storage	4? (unopened)standard stored at -20?, others stored at 4? (opened)
Shipping Condition	4?
Sample Volume	20 µl
Sensitivity	4.22 pg/ml
Standard Curve Range	31.25 - 2000 pg/ml
Spike Recovery Range	86 % - 104 %
Mean Spike Recovery	0.96
CV of Intra plate	8.3 % - 9.2 %
CV of Inter plate	4.8 % - 7.2%
Release Date	43110
Components	96-well polystyrene microplate coated with a monoclonal antibody against SCF Rat SCF Standard, lyophilized SCF Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers
Describtion	This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat SCF. The Rat SCF ELISA is for research use only. Not for diagnostic or therapeutic procedures. Stem Cell Factor (SCF) is a cytokine that binds to the c-Kit receptor. SCF can exist both as a transmembrane protein and a soluble protein. SCF promotes mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response. SCF acts in a synergistic manner with various growth factors, such as IL-3, IL-6, IL-11, GM-CSF, G-CSF, and Epo to induce myeloid and erythroid lineage colony formation. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. Cardiomyocyte-specific overexpression of transmembrane SCF promotes stem cell migration and improves cardiac function and animal survival after myocardial infarction. Mutations in this gene in dogs suggest it is a candidate skin cancer susceptibility gene.