Mouse M-CSF ELISA Kit Plate

Product Detail

Brand	MultiSciences
CatNum	70-EK2144
ProductName	Mouse M-CSF ELISA Kit
CustomsName	Mouse M-CSF ELISA Kit
Application	ELISA
Reactivity	Mouse
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, cell culture supernates
Format	96-well strip plate
Storage	4? (unopened) standard stored at -20?, others stored at 4? (opened)
Shipping Condition	4?
Sample Volume	20 µl
Sensitivity	1.89 pg/ml
Standard Curve Range	31.25 - 2000 pg/ml
Spike Recovery Range	90 % - 108 %
Mean Spike Recovery	1.01
CV of Intra plate	4.6 % - 7.0%
CV of Inter plate	3.2 % - 4.7 %
Components	96-well polystyrene microplate coated with a monoclonal antibody against IFN-? Mouse IFN-? Standard, lyophilized IFN-? Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers
Describtion	This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse M-CSF. The Mouse M-CSF ELISA is for research use only. Not for diagnostic or therapeutic procedures. Macrophage colony-stimulating factor (M-CSF), also known as colony stimulating factor 1 (CSF1), is a secreted cytokine released by osteoblasts. It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis. M-CSF has been described to play a role in renal pathology including Acute kidney injury (AKI) and Chronic Renal Failure (CRF). The chronic activation of monocytes can lead to multiple metabolic, hematologic and immunologic abnormalities in patients with CRF. In the context of AKI, M-CSF has been implicated in promoting repair following AKI, but also been described in an opposing role, driving proliferation of a pro-inflammatory macrophage phenotype.