Human Apolipoprotein AII/Apo A2 Antibody ELISA Kit

Product Detail

FactoryName	Multisciences
CatNum	EK1258
ProductName	Human Apolipoprotein AII/Apo A2 ELISA Kit
CustomsName	Human Apolipoprotein AII/Apo A2 ELISA Kit
Application	ELISA
Reactivity	Human
SDS	SDS - EK1258
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, cell culture supernates
Format	96-well strip plate
Storage	4? (unopened)standard stored at -20?, others stored at 4? (opened)
Shipping Condition	4?
Sample Volume	10 µl (prediluted)
Sensitivity	0.53 pg/ml
Standard Curve Range	7.81 - 500 pg/ml
Spike Recovery Range	91% - 123%
Mean Spike Recovery	106%
CV of Intra plate	3.8% - 4.5%
CV of Inter plate	4.3% - 9.4%
Components	96-well polystyrene microplate coated with a monoclonal antibody against Apo A2 Human Apo A2 Standard, lyophilized Apo A2 Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers
Describtion	This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Apo A2. The Human Apolipoprotein AII/Apo A2 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Apolipoprotein AII (Apo A2), the second most abundant protein after Apo A1 of human plasma high-density lipoproteins (HDL), is the most lipophilic of the exchangeable apolipoproteins. Apo A2 is synthesized by the liver and the small intestine and found in plasma as a monomer, dimer. TheApo A2gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Apo A2 may stabilize HDL structure by its association with lipids, and affect the HDL metabolism. Role of Apo A2 in lipid metabolism and atherosclerosis show important clinical significance. Apo A2 levels have been associated with increased susceptibility to atherosclerosis, increased free fatty acid levels, increased body fat, and increased insulin resistance.